14-26 Sysmex XP-300
Technical Information
Revised October 2012
RBC/PLT analysis flow
In RBC/PLT analysis, RBC and platelet count in the blood are measured. The flow of RBC/PLT
analysis is described below:
• Whole Blood Mode
(1) Blood is aspirated from the sample probe into the SRV.
(2) 4.0 µL of blood measured by the SRV is diluted into 1:500 with 1.996 mL of diluent and brought
to the mixing chamber as diluted sample. (1st step dilution)
(3) Out of the 1:500 dilution sample, 40 µL is measured by the SRV, diluted into 1:25,000 with
1.960 mL of diluent, then transferred to the RBC/PLT TD chamber. (2nd step dilution)
(4) 250 µL of the sample in the RBC/PLT TD chamber is aspirated through the aperture. At this
time, RBC and PLT are counted by the DC detection method.
At the same time, HCT (hematocrit value) is calculated by RBC pulse height detection method.
Tube
(Whole Blood: 50 µL)
Mixing Chamber
Diluent: 2.0 mL
SRV
(1:500 Diluted Sample: 40 µL)
Diluent 1.996 mL
Whole Blood 4 µL
Diluent 1.960 mL
1:500 Diluted Sample 40 µL
RBC/PLT Transducer
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