Sysmex XP-300 14-25
Technical Information
Revised October 2012
• Pre-diluted Mode
(1) Blood sample that was diluted beforehand to 1:26 dilution using CELLPACK. This sample is
aspirated from the sample probe into the SRV.
(2) 78 µL of diluted blood measured by the SRV is transferred to the WBC TD chamber along with
1.922 mL of diluent. At this time, 1.0 mL of WBC/HGB lyse is added to prepare 1:1,000
dilution sample.
When the solution is made to react in this status for approximately 10 seconds, RBC is
hemolyzed and platelets shrink, with WBC membrane held as they are. At the same time,
hemoglobin is converted into red colored methemoglobin.
(3) Of the diluted/hemolyzed sample in the WBC TD chamber, approximately 1 mL is transferred
to the HGB flow cell.
(4) 500 µL of sample in the WBC TD chamber is aspirated through the aperture. The pulses of the
blood cells when passing through the aperture are counted by the DC detection method.
(5) In the HGB flow cell, 555 nm wavelength beam irradiated from the light emitting diode (LED)
is applied to the sample in the HGB flow cell. Concentration of this sample is measured as
absorbance. This absorbance is compared with that of the diluent alone that was measured
before addition of the sample, thereby calculating HGB (hemoglobin value).
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