14-24 Sysmex XP-300
Technical Information
Revised October 2012
CBC analysis
WBC/HGB analysis flow
In WBC and HGB analysis, the volume of WBC and hemoglobin in the blood are measured. The flow
of WBC/HGB analysis is described below:
• Whole Blood Mode
(1) Blood is aspirated from the sample probe into the SRV.
(2) 6 µL of blood measured by the SRV is transferred to the WBC TD chamber along with 1.994
mL of diluent. At the same time, 1.0 mL of WBC/HGB lyse is added to prepare 1:500 dilution
sample.
When the solution is made to react in this status for approximately 10 seconds, RBC is
hemolyzed and platelets shrink, with WBC membrane held as they are. At the same time,
hemoglobin is converted into red colored methemoglobin.
(3) Of the diluted/hemolyzed sample in the WBC TD chamber, approximately 1 mL is transferred
to the HGB flow cell.
(4) 500 µL of sample in the WBC transducer is aspirated through the aperture. The pulses of the
blood cells when passing through the aperture are counted by the DC detection method.
(5) In the HGB flow cell, 555 nm wavelength beam irradiated from the light emitting diode (LED)
is applied to the sample in the HGB flow cell. Concentration of this sample is measured as
absorbance. This absorbance is compared with that of the diluent alone that was measured
before addition of the sample, thereby calculating HGB (hemoglobin value).
Diluent: 2.0 mL
SRV
Tube or Micro tube
Whole Blood Mode: 50 µL
Pre-diluted Mode: 1:26 Diluted Sample 200 µL
Whole Blood Mode: Diluent 1.994 mL
Whole Blood 6 µL
Pre-diluted Mode: Diluent 1.922 mL
1:26 Diluted Sample 78 µL
WBC/HGB Lyse:
Whole Blood Mode 1.0 mL
Pre-diluted Mode 1.0 mL
WBC Transducer
HGB Flow Cell
Whole Blood Mode 1 mL
Pre-diluted Mode 1 mL
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