Olympus FLUOVIEW FV300 - Image Acquisition in XY Observation Mode

APPLIED OPERATIONS/Image Acquisition
IV. OPERATION INSTRUCTIONS
IV . 2-8
Page
2-2-1 Image Acquisition in X Y Observation Mode
This section describes the basic operation procedure from the system configuration to the
image acquisition in the XY observation mode and image saving in a file as shown in the
following chart. The details of each operation will be described in the subsequent
sections.
Save the image.
(Section 2-3-1 in [OPERATION])
Configure the microscope and scan
unit.
(Sections 2-2-1-1 & 2-2-1-2 in [OPERATION])
Set the zoom ratio to 1X.
(Section 2-2-1-3-2 in [OPERATION])
Set the dyeing method.
(Section 2-2-1-1 in [OPERATION])
Set the channel to be acquired.
(Section 2-2-1-3-3 in [OPERATION])
Set the highest scan speed.
(Section 2-2-1-3-4 in [OPERATION])
Perform repeated scanning.
(Section 2-2-1-3-6 in [OPERATION])
Set the XY observation mode.
(Section 2-2-1-3-5 in [OPERATION])
Set a lower scan speed.
(Section 2-2-1-3-10 in [OPERATION])
Re-adjust the image
brightness.
(Section 2-2-1-3-9 in [OPERATION])
Stop repeated scanning.
(Section 2-2-1-3-11 in [OPERATION])
If the image
does not
become
clean
Acquire the image
(Section 2-2-1-4 in [OPERATION])
If the image
becomes
clean
Set the objective magnification.
(Section 2-2-1-3-1 in [OPERATION])
Set the observation range.
(Section 2-2-1-3-8 in [OPERATION])
Adjust the image brightness.
(Section 2-2-1-3-9 in [OPERATION])
Adjust the Z-position to observe the
desired multiple sections.
(Section 2-2-1-3-7 in [OPERATION])