Getting Started FLUOVIEW/Outline of LSM Observation Procedures
IV.
OPERATION INSTRUCTIONS
IV
. 1-40
Page
1-2-6 Selecting the CONFOCAL APERTURE
Set the CONFOCAL APERTURE knob (1) to select the optimum confocal aperture
number for the objective, that is displayed on the control panel.
(Refer to section 1-1-1, “Scan Unit” in this volume.)
1-2-7 Selecting the DETECTION MODE
Set the DETECTION MODE slider to the optimum position according to the dye used
with the observed specimen.
If the optimum setting cannot be identified, see section 1-3, “Online Help” in this volume
and follow instructions in the [Microscope Configuration] window.
Reference Examples
Dye in Use Detected Channels DETECTION MODE slider
FITC
Lucifer Yellow, etc.
CH1
(Pushed-in position, mirror)
FITC + TRITC
FITC + PI, etc.
CH2
TRITC, PI
CH1/CH2
(pushed-in half way to the
position,SDM560)
FITC + CY5
TRITC+CY5
CH1/CH2
CY5, etc. CH2
*(Pulled-out position,
SDM630)
(See Section 1-1-1, “Scan Unit” in this volume.)
1-2-8 Setting the Barrier filters
Engage barrier filters in the light path according to the dye used with the observed
specimen. (For the barrier filter selection method, see section 1-1-1, “Scan Unit” in this
volume.)
The barrier filters are engaged in the light path when the BARRIER FILTERS slider (3) is
set to the pushed-in position and disengage when the switch is set to the pulled-out
position.
If the slider switch is set pushed-in harf way to the position by mistake,
no images can be observed at all.
NOTE
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