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Olympus FLUOVIEW FV300 - Image Acquisition in the Laser Excitation Mode

Olympus FLUOVIEW FV300
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APPLIED OPERATIONS/Image Acquisition
IV.
OPERATION INSTRUCTIONS
IV .
2-92
Page
2-2-12 Image Acquisition in t he Laser Excitation Mode
When you use the FV system with AOTF (FV5-COMBA), the function described in this
section is available.
In the FV system with AOTF, it is possible to cut excitation of laser except the region
where scanning is performed. Moreover, it is also possible to set up a region of laser
excitation to excite laser only to the target part.
REX represents the “region of laser excitation” in this section.
Three kinds of the laser excitation mode, Disable, REX, and Bleach, are selectable
according to your purpose. And each mode can be switched in the XYT observation. In
use of the REX or Bleach mode, a setup of REX mask file is required.
Disable mode: An ordinary mode to acquire an image of specified size.
REX mode: The mode which sets the REX mask file to excite laser only to the target part
and acquire image. The same laser setup as Disable mode is applied.
Bleach mode: The REX mask file other than that of the REX mode can be set. And each
laser setup can also be obtained apart from the Disable/REX mode.
Sets the laser intensity
Switches the laser excitation
modes
TIP

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