APPLIED OPERATIONS/Image Acquisition
IV.
OPERATION INSTRUCTIONS
IV .
2-74
Page
2-2-8 Image acquisition to prevent crosstalk between fluorescence (Sequential
Scan)
An image which is suppressed crosstalk can be acquired sequentially by combination of
excitation laser and those image acquisition channel.
With this image capturing method, the image of a multiple-dyed specimen can be
obtained by sequentially acquiring image slice of each type of fluorescence.
1. Select the [Surface XY-Norm] option button in the [Mode] group box in the
[Acquire] panel and select <Sequen> from the list displayed below it.
Selecting <Sq Clp>, <Sq Rct>, or <Line Sq> enables the image acquisition of the
desired rectangle area, that of the rectangle area at desired angle, or a line
sequential scan.
Line Sequential Scan
Ordinary sequential scan is performed for every one frame for every group,
whereas line sequential scan is performed for every one line for every group.
Therefore, line sequential scan is of advantage in minimizing the time lag
between wavelength and acquiring a fewer cross-talk image.
Image acquisition in the line mode can be performed when
you use the FV300 system with AOTF (FV5-COMBA).
2. Select the observation mode with the option buttons in the [Acquire] panel.
NOTE
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