Olympus FLUOVIEW FV300 - Configuring the Microscope

APPLIED OPERATIONS/Image Acquisition
IV. OPERATION INSTRUCTIONS
IV . 2-9
Page
2-2-1-1 Configuring the Microscope
Set the light path so that the image can be observed through the microscope.
1. Display the [Acquire] panel.
Fig. 2-1 [Acquire] Panel
<Focus> button
The repetition images
can be acquired at a high speed.
Use this button to find an optimum image.
<Once> button
A
cquires an image in the currently
selected observation mode and display
the image in the [Live] panel.
[Settings], [Z Stage], [Time Series],
[Dyes] and [Lasers] sub-panels
These panels are used to set the
information required for image
acquisition.
<XY Repeat> button
Repeats XY scanning to display images
successively in the [Live] panel.
Option buttons
Check a button to select one of the
displayed observation modes.
[Scan Speed] group box
Sets the scan speed. Sliding
to the
left of the scale increases the scan speed
and to the right decreases it.
[PMT], [Gain] and [Offset] LED
sliders
These can be adjusted
independently.
Increasing the PMT voltage improves
the sensitivity.
Increasing the Gain brightens the
image.
[Ch1], [Ch2] and [Ch3] check boxes
Check to select the image acquisition
channel.
Select the magnification of the
objective on the microscope.
The acquired image is displayed.
<Load> button
Loads the observation conditions
at image acquisition.
<Save> button
Saves the observation conditions
at image acquisition.