Olympus FLUOVIEW FV300 - Acquiring Image; Acquiring Image in Accumulation Mode

APPLIED OPERATIONS/Image Acquisition
IV. OPERATION INSTRUCTIONS
IV . 2-22
Page
11 Stopping Repeated Scan ning
1. After the brightness and gain have been adjusted, select the <STOP SCAN>
button in the [Acquire] panel to stop scanning temporarily.
2-2-1-4 Acquiring Image
1. Select the <Once> button. The acquired image will be displayed in the [Live] panel.
2-2-1-5 Acquiring Image in Accumulation Mode
When the image is dark or noisy, use an accumulation mode in image acquisition to
improve the image quality.
Kalman Accumulation and Peak Accumulation
z The Kalman accumulation acquires images for the specified number of times while
averaging the images. This operation is effective for reduction of noise.
z The Peak accumulation acquires images for the specified number of times while adding
the images, and stops image acquisition when any intensity value on the image reaches
the peak (4095). This operation is effective for with the dark lower part in the XYZ
observation and observation of an extremely dark image.
Kalman Accumulation Algorithm
Every time an image is acquired, the pixel values are rewritten based on the following
formulae, where it is assumed that;
n: number of image acquisitions;
l(n): Result of n-times of Kalman accumulations (Intensity values of pixels);
l(new): New intensity value obtained after every image acquisition.
The result of the first Kalman accumulation is identical to the result of ordinary image
acquisition:
l(1) = l(new)
The result of the n-th (n > 1) Kalman accumulation is:
l(n) = (l(n-1) * (n+1) + l(new)) / n
<Once> button