Olympus FLUOVIEW FV300 - Transmitted Image

APPLIED OPERATIONS/Image Acquisition
IV. OPERATION INSTRUCTIONS
IV . 2-63
Page
2-2-3-3 Transmitted Image
Images obtained by transmitted light observation can also be acquired or observed
simultaneously with images obtained by fluorescence observation.
z Set the [DETECTION MODE] lever of the scan unit as shown below.
FL -: Fluorescence observation with monochrome or dual-fluorochrome dyeing
(Ch1), and transmitted light observation (Ch3).
- FL: Fluorescence observation with monochrome or dual-fluorochrome dyeing
(Ch2), and transmitted light observation (Ch3).
FL FL: Fluorescence observations with dual-fluorochrome (Ch1, Ch2), and
transmitted light observation (Ch3).
For the difference between Ch1 and Ch2, see sections 2-2-3-1,
“Monochrome Image” and 2-2-3-2, “Dual-Fluorochrome Image”.
z When observing fluorescence images simultaneously, set the dyeing method.
1. From the page tabs on the bottom right of the [Acquire] panel, select the
[Dyes] sub-panel.
Fig. 2-20 [Dyes] Sub-panel
2. Select each of the desired specimen dyeing methods in the [Available Dyes]
list box in the [Selected Dyes] group box, and drag the selected method into
the field immediately above the list box.
3. Click the <Apply> button to apply the selected dyeing methods to the [Ch1]
and [Ch2] group boxes on the upper part of the [Acquire] panel.
TIP
[Available Dyes] list box
Lists the available dyes. Select the
desired items from this list and drag
them to the field above it to select the
dyeing method.
<Clear> button
Clear the set dyeing method.
<Prev.> button
Sets the dyeing method which was
set last time by clicking the <Apply>
button.
<Apply> button
A
pplies the dyeing method
selected in the [Selected Dyes]
group box to [Ch1] or [Ch2] in
the [Acquire] panel.
Place the pointer on the
icon displayed in the
[Selected Dyes], and the
dyeing method is shown in
the pop-up display.
[Assign dyes manually] check box
Checking this enables the manual
setting. Dragging the dyeing method in
the list directly to the [Ch] group box
assigns the dye to the desired channel.