Olympus FLUOVIEW FV300 - Dual-Fluorochrome Image

APPLIED OPERATIONS/Image Acquisition
IV. OPERATION INSTRUCTIONS
IV . 2-60
Page
2-2-3-2 Dual-Fluorochrome Image
The wavelength obtained by dual-fluorochrome dyeing can be acquired and observed as
images of the channels (Ch1 or Ch2).
The subsequent description deals with the differences in operation between the
monochrome dyeing and dual-fluorochrome dyeing.
When the specimen dyed by dual-fluorochrome dyeing is observed, the image of
wavelengths below 570 nm is acquired in channel 1 (Ch1) and the image of
wavelengths above 570 nm is acquired in channel 2 (Ch2).
z Set the [DETECTION MODE] lever of the scan unit to “FL FL”.
z Set the two dyeing methods.
1. From the page tabs on the bottom right of the [Acquire] panel, select the [Dyes]
sub-panel.
Fig. 2-19 [Dyes] Sub-panel
2. Select each of the desired specimen dyeing methods in the [Available Dyes] list
box in the [Selected Dyes] group box, and drag the selected method into the field
immediately above the list box.
3. Click the <Apply> button to apply the selected dyeing method to the
[Channel1]/[Channel 2] group box on the upper part of the [Acquire] panel.
[Available Dyes] list box
Lists the available dyes. Select the
desired items from this list and drag
them to the field above it to select the
dyeing method.
<Clear> button
Clear the set dyeing method.
<Prev.> button
Sets the dyeing method which was
set last time by clicking the
<Apply> button.
<Apply> button
A
pplies the dyeing method
dragged in the [Selected
Dyes] group box to the
[Channel 1]/[Channel 2]
group box in the [Acquire]
panel
Place the pointer on the
icon displayed in the
[Selected Dyes], and the
dyeing method is shown in
the pop-up display.
[Assign dyes manually] check box
Checking this enables the manual
setting. Dragging the dyeing method in
the list directly to the [Ch] group box
assigns the dye to the desired channel.