Olympus FLUOVIEW FV300 - Setting the Dyeing Methods

Getting Started FLUOVIEW/Outline of LSM Observation Procedures
IV. OPERATION INSTRUCTIONS
IV . 1-38
Page
1-2-5 Setting the Dyeing Methods
1. From the page tabs on the bottom right of the [Acquire] panel, select the [Dyes]
sub-panel.
2. Select the specimen dyeing method by dragging [FITC] and [TRITC] in the [Available
Dyes] list box in the [Selected Dyes] group box to the field immediately above the
list box.
3. Click the <Apply> button to apply the selected dyeing method to the [Channel1] /
[Channel 2] group box on the upper part of the [Acquire] panel.
When the dyeing method is selected in the [Available Dyes] list box and the
<Apply> button is clicked, the dyeing method will be set automatically to the
optimum channels.
The confocal aperture is also set automatically to the optimum channel for
the wavelength and the objective being selected in the [Acquire] panel.
[Available Dyes] list box
Lists the available dyes. Select the
desired items from this list and drag
them to the field above it to select the
dyeing method.
<Clear> button
Clear the set dyeing method.
<Prev.> button
Sets the dyeing method
which was set last time by
clicking the <Apply> button.
<Apply> button
A
pplies the dyeing method dragged in
the [Selected Dyes] group box to the
[Channel 1]/[Channel 2] group box in
the
[
Ac
q
uire
]p
anel.
TIP
Place the pointer on the icon displayed
in the [Selected Dyes], and the dyeing
method is shown in the pop-up display.
[Assign dyes manually] check box
Checking this enables the manual
setting. Dragging the dyeing method in
the list directly to the [Ch] group box
assigns the dye to the desired channel.