Olympus FLUOVIEW FV300 - Brightness Overlap Level between 2 Channels (Colocalization)

APPLIED OPERATIONS/Image Processing
IV. OPERATION INSTRUCTIONS
IV . 2-245
Page
2-6-4 Brightness overlap level between 2 channels (Colocalization)
This function enables to observe overlap intensity of two channel of image.
1. Acquires or opens images which more than two channels, and displays the images
on [Display] panel.
2. On the [Display] panel, displays and overlaps 2 channel image that to be observed.
See section 2-4-4-2 for overlapping the image display.
3. On the overlapped images, click right-mouse button to show the following pop up
menu.
4. Select [Install Colocalization: .v ] of [ViewProcessor]. Then [Colocalization
Processor] appears.
[] shows the dyeing method or its number of channel.
There are the following 4 Annotation Mode in [Colocalization Processor] dialog box.
x None Mode
x Threshold Mode
x Min-Max Bounds Mode
x Regions Mode