Olympus FLUOVIEW FV300 - Default Chapter; Troubleshooting Guide

TROUBLESHOOTING GUIDE
VI . TROUBLESHOOTING
VI. 1-1
Page
1 TROUBLESHOOTING GUIDE
Under certain conditions, performance of this system may be adversely affected by factors other than
defects. If a problem occurs, please review the following list and take remedial action as needed.
If you cannot solve the problem after checking the entire list, please contact your local Olympus
representative for assistance.
Before contacting Olympus, please fill the “Inquiry Table” at the end of this volume and inform Olympus of
its contents.
Phenomenon Cause Treatment Manual Ref. Pages
1. Fluorescence image
cannot be observed.
Laser is not oscillating. Turn the laser unit ON.
Ensure that the emission key
is set to the ON position.
1-2-1, OPERATION
INSTRUCTIONS
The light path selector knob
of the microscope (or light
path selector button on an
inverted microscope) is not
set to the LSM light path.
Set the light path selector to
the LSM light path.
1-1-2 & 1-2-4,
OPERATION
INSTRUCTIONS
An excitation tube or
analyzer for visual
observation is engaged in
the microscope.
Disengage the excitation
tube and analyzer from the
light path.
1-2-4, OPERATION
INSTRUCTIONS
The objective is not engaged
in the light path.
Stop the objective at a click
position.
Instruction manual
of microscope
The light path is blocked by
the confocal aperture knob.
Stop the confocal aperture
knob at a click position.
1-1-1, 1-2-6,
OPERATION
INSTRUCTIONS
The confocal aperture
number is small.
Increase the confocal
aperture.
1-1-1, OPERATION
INSTRUCTIONS
The light path is blocked by
barrier filters.
Push the barrier filters into
the specified position.
1-1-1 & 1-2-8,
OPERATION
INSTRUCTIONS
The light path is blocked by
the light path detection
switch.
Stop the light path detection
switch at a click position.
1-1-1 & 1-2-7,
OPERATION
INSTRUCTIONS
The fluorescent dye and
excitation wavelength does
not match.
Select a laser suitable for the
fluorescent dye method.
See 2-2-1-2, “Configuring
the Scan Unit” in
OPERATION
INSTRUCTIONS and follow
instructions of the
[Microscope Configuration]
window.
1-3-2 & 2-2-1-2,
OPERATION
INSTRUCTIONS