APPLIED OPERATIONS/Image Acquisition
IV.
OPERATION INSTRUCTIONS
IV .
2-77
Page
2-2-8-1 Virtual Channel Function
The virtual channels refer to the simulated detection channels produced when a single
detector switched in sequential scan. The virtual channels make it possible to perform
sequential scan by switching the lasers and filters according to the preset condition by
increasing the number of detection channels. (With the FV300, they should be switched
manually.)
Operation Example
The following procedure deals with the case of observing FITC in CH1, PI in CH2 and
Cy5 in a virtual channel.
1. In the [Dyes] sub-panel of the [Acquire] panel, select the dyeing methods and click
the <Apply> button. (Example: FITC and PI are selected.)
For details, see step 2 in the procedure in section 2-2-1-1, “Configuring the
Microscope”.
2. Click the <Once> button to acquire an image.
<Once> button
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