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Olympus FLUOVIEW FV300 - Page 190

Olympus FLUOVIEW FV300
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APPLIED OPERATIONS/Image Acquisition
IV.
OPERATION INSTRUCTIONS
IV .
2-76
Page
Fig. 2-23 [Lasers] Sub-panel
7. Among the laser <Off> buttons on the right of the [Laser Intensity] scales in the
[Lasers] group box, click the <Off> button of the laser you want to use in
transmitted observation. This should change the <Off> button to the <Rdy> button
(displayed in the pressed-in condition).
8. Click the <OK> button in the [FLUOVIEW] dialog box.
Image acquisition starts.
9. Adjust the brightness with the [PMT], [Offset] and [Gain] LED sliders in the [Ch]
group box in the [Acquire] panel. See section 2-2-1-3-9, “Adjusting the Image
Brightness” for details.
10. After image acquisition, select <Normal> under the [Surface XY] button in the
[Mode] group box in the [Acquire] panel to return from the image acquisition mode.
<Off> button
Select the laser type.

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