Getting Started FLUOVIEW/Outline of LSM Observation Procedures
IV.
OPERATION INSTRUCTIONS
IV.
1-43
Page
1-2-9 Setting the Excitation DM
Using the excitation DM selector knob (4), select the excitation DM according to the
wavelength of laser in use.
1-2-10 Setting the ND Filters of the Laser Combiner
When you use the laser combiner, you can set the laser intensity by setting ND filter on
the laser combiner.
Display the [Acquire] panel.
Set each laser intensity by sliding the scale bar in the [Laser Intensity] group box of the
[Lasers] sub-panel, in accordance with specimen’s brightness, fluorescence crosstalk
and photo-bleaching.
While using the HeNe green laser, try out the laser power 50% by setting the [Intensity]
scale bar in the [Laser Intensity] group box.
For other lasers, try the laser power 10%.
[Laser Intensity] group box
Set the laser intensity value by
the scale bar..
The number of the displayed
laser intensity sliders varies
depending on that of channels
setting for the acquisition.
(4) Excitation DM selector knob
(1) CONFOCAL APERTURE
knob
(2) DETECTION MODE
slider
(3) BARRIER FILTERS slider
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