Olympus FLUOVIEW FV300 - Annotation Mode

APPLIED OPERATIONS/Image Processing
IV. OPERATION INSTRUCTIONS
IV . 2-246
Page
2-6-4-1 Annotation Mode
1 None Mode
None Mode is the default of [Colocalization Processor] dialog box. The mode can be
changed by option button in [Annotation Mode] Group Box. The items described for this
mode can be used in other modes.
Fig. 2-99 [Colocalization Processor] Dialog Box
In case of Annotation Mode is [None]
.
[Data Selection] Group Box
<Disabled> Option Button
Graph and Text box will disappear from
[Colocalization Processor] dialog box and
<Hide> button at lower right will change to
<Close> button. When <Close> is clicked,
the [Colocalization Processor] dialog will
dismiss.
<Current slice> Option Button
This button executes colocaliztion for
images displayed on 䌛Display䌝 panel.
[Histogram] Graph
It acquires brightness value of 1st channel
selected on horizontal axis and brightness of
value of other channel on vertical axis and
displays brightness distribution. The point with
the value which less than [LUT min] text box is
colored with blue, and the point more than
[LUT max] value is colored with in red; and
other point will be colored by gray scale.
[Summary] Text Box
Summary : Dye method of vertical and
horizontal axis or channel number
Mode : Option button selected at [Data
Selection] group box
Samples processed : Total numbers o
f
pixels on whole image to be processed
Binning : [Bin Width] Setting condition o
f
text box
Max. samples in any bin : Max. value in
plot data on graph
[Annotation Mode] Group Box
Select either [None], [Threshold], [Min-
Max Bounds] or [Regions] option button.
In case of [Threshold], [Min-Max Bounds]
or [Regions] are selected, text box
appears in [Annotation] Group Box.
[Colocalization Processor] is displayed,
[None] is selected.
<Accept Histogram> Button
[Histogram] graph appears in [Display]
panel.
The graph can be handled same with the
acquired image.
[LUT Max] Text Box
Double-click inside text
box so that the display
can alternately be
changed in two methods.
1. All data uniform
display
2. Binar
y
displa
y
o
f
[LUT Min] Text Box
<Hide> Button
It hides [Colocalization
Processor] dialog box.
The [Colocalization
Processor] dialog box
appears again by pressing
<Colocalization
Processor> button in
[Display] panel.
[Zoom] Text Box
It sets display
magnification of
[Histogram] graph.
[Bin Width] Text Box
It sets resolution of
[Histogram] graph. For
example, if the value is
16, it displays
brightness in 16 gray-
scale as one plot.