Olympus FLUOVIEW FV300 - Example of Assembling a Protocol; Example of Setting Procedure of FRAP Observation

APPLIED OPERATIONS/ Protocol processor
IV. OPERATION INSTRUCTIONS
IV . 2-176
Page
2-4-8 Example of assembling a protocol
This section describes an example of assembling a protocol.
1 Example of Setting Procedure of FRAP Observation
An example of editing a protocol is described below assuming the FRAP observation.
Set Zoom
ratio to 2
An image
acquisition
Wait fo
r
5 sec.
Image acquisition:
3 frames
(Photo bleaching)
Protocol
Track 1 Track 2
Track 3
Set Zoom
ratio to 10
Set Zoom
ratio to 2
Image
ac
q
uisition
Image
saving
Repeat 30 times
Image
ac
q
uisition
Wait for
5 sec.
Initial image acquisition: Track 1 sets the zoom ratio to 2 x, and acquires an image.
Photobleaching: Track 2 sets the zoom ratio to 10 x, and acquires image 3 times to
make photobleach on the specimen.
Recovery measurement: Track 3 sets the zoom ratio to 2 x, and acquire image 3 times
with 5 seconds interval to observe the recovery. Then, stores the
image.
Execute a XY repeated scanning and adjust the set values for image
acquisition before executing the protocol.
1-㧝 Setting the observation condition
Before setting and starting the FRAP observation using the Protocol Processor, execute
a repeated scan and set the condition for the observation.
Assuming FITC to Channel 2 and image size to 320X240 pixels, the following figure
shows an example of image acquisition.
TIP