Getting Started FLUOVIEW/Outline of LSM Observation Procedures
IV.
OPERATION INSTRUCTIONS
IV
. 1-34
Page
1-2-4-3 Combination with Inverted Microscope (IX81)
1. From the page tabs on the bottom right of the [Acquire] panel, select the [Settings]
sub-panel.
Fig. 1-5 [Settings] Sub-panel
2. Select the <LSM> button in the [Light Path] group box.
The <LSM> button looks pushed in to indicate that it is selected.
(When scanning is started while the <BI> button is selected, the LSM light path is
selected automatically. It is switched back to the visual observation automatically
when scanning completes.)
3. Push the hand switch button (6) to set reflected light fluorescence unit to
.
4. When the IX2-MDICT analyzer (3) is in use, disengage it from the light path by
pressing the button (6) on the U-HSTR2 hand switch.
5. When a fluorescence observation alone is required, disengage the U-DICTS
transmitted DIC slider (3) by setting the switch to the pulled-out position. When a
transmitted light DIC observation or a simultaneous fluorescence & transmitted light
DIC observation is required, engage the U-DICTS and the optimum transmitted DIC
slider for the objective into the light path by operating the universal condenser (4).
In a simultaneous fluorescence & transmitted light differential interference
observation, leaving the U-DICTS within the light path degrades the fluorescence
image resolution to some extent.
6. During the transmitted light observation, be sure to disengage the filter (5) from the
light path.
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