Olympus FLUOVIEW FV300 - Combination with Upright Microscope (BX); Setting the LSM Light Path

Getting Started FLUOVIEW/Outline of LSM Observation Procedures
IV. OPERATION INSTRUCTIONS
IV. 1-31
Page
1-2-4 Setting the LSM Light Path
1-2-4-1 Combination with Upright Microscope (BX)
1. Pull out the light path selector on the trinocular tube fully to the stop position.
2. Pull out the light path selector (7) on the TV adapter fully to the stop position.
3. Push the hand switch button to (8) set
to be displayed in the cube display
window (2) on the reflected light fluorescence vertical illuminator (when using the
Motorized microscope).
Rotate the cube turret (2) on the reflected light fluorescence vertical illuminator to
when using the Manual microscope).
4. When the U-MDICT3 analyzer (3) is in use, disengage it from the light path by
setting the switch.
5. When only fluorescence observation is required, disengage the U-DICTHR/WI-
DICTHRA transmitted light DIC (4) by setting the switch to the pulled-out position.
When transmitted light differential interference observation or simultaneous
fluorescence + transmitted light differential interference observation is required,
engage the transmitted light DIC and the optimum transmitted light DIC in the light
path by operating the universal condenser (6).
6. For transmitted light observation, disengage any filter (5) from the light path.
When you perform transmitted observation using laser with BX61WI, use the filter
knob to disengage the LBD from the light path and engage the FR (Frost) into the
light path. Disengaging the FR (Frost) from the light path may generate interference
fringes on an image.