Olympus FLUOVIEW FV300 - XZT Observation Mode

APPLIED OPERATIONS/Image Acquisition
IV. OPERATION INSTRUCTIONS
IV . 2-35
Page
2-2-2-3 XZT Observation Mode
The description in this section will be focused on the image acquisition operations in the
XZT observation mode that are not used in the XY observation modes (which are the
operations enclosed in
in the chart on the next page). For other operations, see
section 2-2-1, “Image Acquisition in XY Observation Mode”. The details of each operation
will be described in the subsequent sections.
Save image.
(Section 2-3-1 in [OPERATION])
Set the channel to be acquired.
(Section 2-2-1-3-3 in [OPERATION])
Set the highest scan speed.
(Section 2-2-1-3-4 in [OPERATION])
Perform repeated scanning.
(Section 2-2-1-3-6 in [OPERATION])
Set the XY observation mode.
(Section 2-2-1-3-5 in [OPERATION])
Set the observation range.
(Section 2-2-1-3-8 in [OPERATION])
Set a lower scan speed.
(Section 2-2-1-3-10 in [OPERATION])
Re-adjust the image brightness.
(Section 2-2-1-3-9 in [OPERATION])
Stop repeated scanning.
(Section 2-2-1-3-11 in [OPERATION])
Set the observation mode.
(Section 2-2-2-3-2 in [OPERATION])
Acquire image.
(Section 2-2-2-3-7 in [OPERATION])
Set the observation line.
(Section 2-2-2-3-3 in [OPERATION])
Set the range of the multiple sections to be
observed (the Z-direction scanning range).
(Section 2-2-2-3-1 in [OPERATION])
Set the numbers of Z-direction steps
and acquired image slices.
(Section 2-2-2-3-4 in [OPERATION])
Adjust the Z-position to observe the
desired multiple sections.
(Section 2-2-1-3-7 in [OPERATION])
Adjust the image brightness.
(Section 2-2-1-3-9 in [OPERATION])
Set the zoom ratio to 1X.
(Section 2-2-1-3-2 in [OPERATION])
Set the interval time.
(Section 2-2-2-3-5 in [OPERATION])
Set the number of scans.
(Section 2-2-2-3-6 in [OPERATION])
Set the dyeing method.
(Section 2-2-1-1 in [OPERATION])
Configure the microscope and scan unit.
(Sections 2-2-1-1 & 2-2-1-2 in [OPERATION])
Set the objective magnification.
(Section 2-2-1-3-1 in [OPERATION])
If the image
does not
become clean
If the image
becomes
clean