APPLIED OPERATIONS/Image Acquisition
IV.
OPERATION INSTRUCTIONS
IV .
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Page
z Engage the filter by setting the [BARRIER FILTERS] lever of the scan unit. (Setting
the lever to “IN” engages the filter.)
Also see section 2-2-1-2, “Configuring the Scan Unit” and follow the instructions
given by the [Microscope Configuration] window.
z Make the two channels ready for image acquisition.
In the [Acquire] panel, make sure that the check box in the [Ch1] and [Ch2] group
boxes that show the dyeing methods are check-marked to indicate that the
corresponding channels are ready for image acquisition.
z Set the image brightness of each channel.
Adjust the brightness of the image of each channel by using the [PMT], [Offset] and
[Gain] LED sliders in the [Acquire] panel. For details, refer to section 2-2-1-3-9,
“Adjusting the Image Brightness”.
Ch2 check box
Ch1 check box