APPLIED OPERATIONS/Image Acquisition
IV.
OPERATION INSTRUCTIONS
IV .
2-28
Page
Save image.
(Section 2-3-1 in [OPERATION])
Configure the microscope and scan unit.
(Sections 2-2-1-1 & 2-2-1-2 in [OPERATION])
Set the objective magnification.
(Section 2-2-1-3-1 in [OPERATION])
Set the dyeing method.
(Section 2-2-1-1 in [OPERATION])
Set the channel to be acquired.
(Section 2-2-1-3-3 in [OPERATION])
Set the highest scan speed.
(Section 2-2-1-3-4 in [OPERATION])
Perform repeated scanning.
(Section 2-2-1-3-6 in [OPERATION])
Set the XY observation mode.
(Section 2-2-1-3-5 in [OPERATION])
Set the observation range.
(Section 2-2-1-3-8 in [OPERATION])
Set a lower scan speed.
(Section 2-2-1-3-10 in [OPERATION])
Re-adjust the image brightness.
(Section 2-2-1-3-9 in [OPERATION])
Stop repeated scanning.
(Section 2-2-1-3-11 in [OPERATION])
Set the observation mode.
(Section 2-2-2-1-2 in [OPERATION])
Acquire image.
(Section 2-2-2-1-5 in [OPERATION])
Set the observation line.
(Section 2-2-2-1-3 in [OPERATION])
Set the range of the multiple sections
to be observed (the Z-direction
scanning range).
(Section 2-2-2-1-1 in [OPERATION])
Set the numbers of Z-direction steps
and acquired image slices.
(Section 2-2-2-1-4 in [OPERATION])
Adjust the Z-position to observe the
desired multiple sections.
(Section 2-2-1-3-7 in [OPERATION])
Adjust the image brightness.
(Section 2-2-1-3-9 in [OPERATION])
Set the zoom ratio to 1X.
(Section 2-2-1-3-2 in [OPERATION])
If the image
does not
become
clean
If the image
becomes
clean
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