APPLIED OPERATIONS/Image Acquisition
IV.
OPERATION INSTRUCTIONS
IV .
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Page
z Make channel ready for transmitted image acquisition.
In the [Acquire] panel, make sure that the [Transmitted] check box in the [Ch3]
group box is check-marked to indicate that Ch3 is ready for image acquisition.
z When observing a fluorescence image simultaneously, set the required channel
ready for acquisition of fluorescence image.
Make sure that the check box showing the dyeing method in the [Ch1] or [Ch2]
group box is check-marked to indicate that the corresponding channel is ready for
image acquisition.
z Set the brightness of the transmitted image.
Adjust the brightness of the image of Ch3 image by using the [PMT], [Offset] and
[Gain] LED sliders in the [Ch3] group box in the [Acquire] panel. For details, refer to
section 2-2-1-3-9, “Adjusting the Image Brightness” in the [OPERATION] volume.
z When observing a fluorescence image(s) simultaneously, also set the brightness of
the fluorescence image(s).
Adjust the brightness of the image of the transmitted image acquisition channel(s)
by using the [PMT], [Offset] and [Gain] LED sliders in its group box in the [Acquire]
panel. For details, refer to section 2-2-1-3-9, “Adjusting the Image Brightness” in the
[OPERATION] volume.
Ch3 check bo
Ch2 check box
Ch1 check box Ch3 check box
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