APPLIED OPERATIONS/Image Acquisition
IV.
OPERATION INSTRUCTIONS
IV .
2-104
Page
7 Acquiring Data
The experiment data before and after photobleaching of certain region can be obtained
after performing an ordinary image acquisition in the first scan, an image acquisition in
the Bleach mode in the second scan , and an ordinary image acquisition in the third
scan. When the REX mode is selected in the third scan, the data of the region other than
the specified region can not be obtained since the laser is irradiated only to the specified
region. However, the REX mode is effective to prevent fading of the obtained data.
1. Select the [Disabled] option button in the [AOTF] group box in the [Lasers] sub-
panel.
2. Click the <XYT> button to start image acquisition.
3. Select the [Bleach mode] option button in the [AOTF] group box in the [Lasers]
sub-panel.
Select the [Bleach mode] option button within 10 seconds before the
next scanning is started.
In the TIME COURSE software (optional), when the REX mask file in
the REX or Bleach mode is changed while image scanning is
performed obtaining the real time graph, the laser is irradiated while
the pop-up menu is displayed. however, the real time graph is not
plotted. Please be aware this before using.
<XYT> button
[Disabled] option button
[Bleach mode] option button
NOTE
NOTE
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