Olympus FLUOVIEW FV300 - Page 303

APPLIED OPERATIONS/Protocol processor
IV. OPERATION INSTRUCTIONS
IV . 2-189
Page
Set each Track as sown below;
z Setting Track 1
Acquire nine images in XYT observation.
1. Select “New mode”-“XYT” in [Mode] of [Track].
2. Select “Normal” in [Start by] of [Track].
3. Enter “9” to [Condition] of [Track].
4. Select “Middle” in [Scan Speed].
5. Enter the laser intensity value for image acquisition to [Laser Intensity].
6. Enter the PMT value of each channel to [PMT Voltage].
7. Enter image file name to be stored to [Save FileName.]㪅
8. Enter a comment in [Comment] if necessary.
The actual image file name becomes using the name of [Image File Name]
that followed by the acquisition order number.
Assign the image file name in the [Image File Name] text box area before
protocol starts..
z Setting Track 2
Specifies N variable and designate range to be repeated. Protocol continues between
the next Track of “For” statement and the Track just before “Loop” statement.
1. Select “For” in [Mode] of [Track].
2. Enter ”N = 1 To 7” to [Condition] of [Track].
3. It is not necessary to enter the values in other cells.
z Setting Track 3
Acquire nine images at one-hour interval in each Track in XYT observation.
1. Select “New mode”-“XYT” in [Mode] of [Track].
2. Enter “Wait 3600” in [Stand by] of [Track]. (Enter the value by the second.)
3. Enter “9” in [Condition] of [Track].
4. Select “Middle” in [Scan Speed].
5. Enter the laser intensity value for use in image acquisition in the cell for each laser
in [Laser Intensity].
Enter “8” in [Ar] and “50” in [HeNe-G].
TIP