Olympus FLUOVIEW FV300 - Page 362

APPLIED OPERATIONS/Image Processing
IV. OPERATION INSTRUCTIONS
IV . 2-248
Page
[Histogram] graph
Explanation of [Annotation] Text Box
Display in Text Box Explanation
Threshold Annotation Threshold Mode
Total samples in histogram: Total numbers of image pixels to be
processed
(Dye-name1)[Thresh: ] Name of Dye method. Threshold value in X-
axis.
<threshold: Total numbers of image pixels below the
threshold value in X-axis
((A+C)/(A+B+C+D))x100 [%]
>=threshold: Total numbers of image pixels above the
threshold value in X-
axis((B+D)/(A+B+C+D))x100 [%]
(Dye-name2)[Thresh: ] Name of Dye method. Threshold value in Y-
axis
<threshold: Total numbers of image pixels below the
threshold value in Y-axis
((C+D)/(A+B+C+D))x100 [%]
>=threshold: Total numbers of image pixels above the
threshold value in Y-axis
((A+B)/(A+B+C+D))x100 [%]
Upper-left Upper left portion of [histogram] graph
Samples Total numbers of image pixels contained in
upper left portion
[of (Dye-name1) < threshold] (A/(A+C))x100 [%]
[of (Dye-name2) >= threshold] (A/(A+B))x100 [%]
Of all samples (A/(A+B+C+D))x100 [%]
Upper-right Upper right portion of [histogram] graph
Samples Total numbers of image pixels contained in
upper right portion
[of (Dye-name1) >= threshold] (B/(B+D))x100 [%]
[of (Dye-name2) >= threshold] (B/(A+B))x100 [%]
Of all samples (B/(A+B+C+D))x100 [%]
Lower-left Lower left portion of [histogram] graph
Samples Total numbers of image pixels contained in
lower left portion
[of (Dye-name1) < threshold] (C/(A+C))x100 [%]
[of (Dye-name2) < threshold] (C/(C+D))x100 [%]
Of all samples (C/(A+B+C+D))x100 [%]
Lower-right Lower right portion of [histogram] graph
Samples Total numbers of image pixels contained in
lower right portion
[of (Dye-name1) >= threshold] (D/(B+D))x100 [%]
[of (Dye-name2) < threshold] (D/(C+D))x100 [%]
Of all samples (D/(A+B+C+D))x100 [%]
A
B
DC