Olympus FLUOVIEW FV300 - Page 369

APPLIED OPERATIONS/Image Processing
IV. OPERATION INSTRUCTIONS
IV . 2-255
Page
・
Pearson’s Coefficient
：
In case that the value to be acquired is defined as
r
,
¦¦
¦


ii
iiii
i
iiii
FFFF
FFFF
r
2
,2,2
2
,1,1
,2,2,1,1
)()(
)()(
However, legends used for image measurement are as follows:
i
F
,1
: Brightness of wavelength
1
O at i -th pixel,
i
F
,2
: Brightness of wavelength
2
O at
i -th pixel
¦
i
ii
F
N
F
,1,1
1
: Average brightness of wavelength
1
O ,
¦
i
ii
F
N
F
,2,2
1
: Average
brightness of wavelength
2
O
・
Overlap
：
In case that the value to be acquired is defined as
r
,
¦¦
¦


i
i
i
i
i
ii
FF
FF
r
2
,2
2
,1
,2,1
䊶Overlap Index #1
：
In case that the value to be acquired is defined as
1
k ,
¦
¦

i
i
i
ii
F
FF
k
2
,1
,2,1
1
䊶Overlap Index #2
：
In case that the value to be acquired is defined as
2
k ,
¦
¦

i
i
i
ii
F
FF
k
2
,2
,2,1
2
・
Colocalization Index #1
：
In case that the value to be acquired is defined as
1
m ,
¦
¦

i
i
Ai
i
F
F
m
,1
,1
1
However, Ai  should be i -th pixel that belongs to aggregation A that is greater than
threshold value.
・
Colocalization Index #2
：
In case that the value to be acquired is defined as
2
m ,