Olympus FLUOVIEW FV300 - Page 59

Getting Started FLUOVIEW/Outline of LSM Observation Procedures
IV. OPERATION INSTRUCTIONS
IV. 1-21
Page
x Transmitted light observation procedure
Set the observation condition.
If no image is
displayed.
Start the system
x
Turn the system power ON.
(Sec 1-2-1)
x
Start the FLUOVIEW software.
(
Sec 1
-2-2)
If the image is still not displayed
Select the LSM light path.
(Sec 1-2-4)
Select the light path for 100 binocular
tube and focus on the specimen.
(Sec 1-2-3)
When the combination using the laser
combiner is used, set the optimum ND
filters for the laser. (Sec 1-2-10)
x Set the objective magnification.
(Sec 1-2-12-1)
x Set the zoom ratio to 1X.
(Sec 1-2-12-2)
x Set the channels. (Sec 1-2-12-3)
x
Set the highest scan speed.
(Sec 1-2-12-4)
x
Select the XY observation mode.
(Sec 1-2-12-5)
x Perform repeated scanning.
(Sec 1-2-12-6)
If an image is
displa
y
ed
Adjust PMT Voltage.
(Sec 2-2-1-3-9)
Acquire an image.
(Sec 1-2-14)
Save the image.
(Sec 1-2-15)
Exit from the FLUOVIEW software.
(Sec 1-2-16)
Turn the system power OFF.
(Sec 1-2-17)
Image is
displayed in
the [Live]
panel of the
software.
Change the with a filter with higher
transmittance. (Sec 1-2-10)
x
Set the multiple sections to be observed.
(Sec 1-2-12-7)
x Set the area to be observed.
(Sec 1-2-12-8)
x Set a lower scan speed.
(Sec 1-2-12-9)
x Stop repeated scanning.
(Sec 1-2-12-10)