SYSMEX XP Series - Page 262

14-32 Sysmex XP-300
Technical Information
Revised February 2013
3. WBC Histogram Error Flag
When the WBC histogram is normal with 3 peaks, there are a lower trough (T1) and a higher trough
(T2) between LOWER discriminator (LD) and UPPER discriminator (UD).
• Output Example of Normal WBC Three-Peak Histogram
When TROUGH Discriminator (T1) or (T2) cannot be set or when frequency for a set discriminator
position is higher than the range, it is flagged as WBC histogram error. Those histogram error flags
are listed below in the order of higher priority. If more than one flag is applied, the flag of the highest
priority is taken.
WL: Relative frequency for LOWER discriminator (LD) exceeds the range. Probable cause is
inclusion of numerous platelet agglutinations, large platelets, and etc.
T1: Lower TROUGH Discriminator, that distinguishes lymphocytes and mixed cells, cannot be not
determined.
T2: Higher TROUGH Discriminator, that distinguishes mixed cells and neutrophils, cannot be not
determined.
F1: Small cell histogram error. Relative frequency for T1 exceeds the range.
F2: Middle cell histogram error. Relative frequency for T1 or T2 exceeds the range.
F3: Large cell histogram error. Relative frequency for T2 exceeds the range.
WU: Relative frequency for UPPER discriminator (UD) exceeds the range. Applicable case is that in
which considerable leukocyte aggregation or numerous abnormal blood cells are present.
AG: The particle count equal to or less than the LD exceeds a prescribed range. Probable cause is
platelet agglutination, which does not alter WBC count but may result in decreased platelet
count. Therefore, this flag is added to the PLT parameter.
300
WBC
UD
200100
LD T1 T2
[fL]
WBC 6.7 [×10
3
/µL]
LYM% 28.3 [%]
MXD% 17.4 [%]
NEUT% 54.3 [%]
LYM# 1.9 [×10
3
/µL]
MXD# 1.2 [×10
3
/µL]
NEUT# 3.6 [×10
3
/µL]