Table of Contents
Adding Diagonal Separators to Plots 249
Applying a Region to All Plots 250
Adding Annotations to Plots 251
Using Plot Ratios 253
Exporting Plots and Histograms 256
Configuring Instrument Settings 257
PMT and Laser Controls 258
Setting Up Multiple Panels 262
Running or Saving the Experiment 265
Running a Quick (Stat) Sample 266
Adding a Stat Tube to a Plate or Rack 267
Running an Existing Experiment 270
Importing Settings 272
Chapter 8 Acquiring Samples 275
Loading Sample Media 275
Setup Mode Controls 276
Acquiring Initial Sample in Setup Mode 278
Acquisition Mode Controls 282
Running Samples in Acquisition Mode 283
Using High-Throughput Mode 285
Pausing, Stopping, and Resuming Samples and Experiments 286
Pausing Sample Acquisition 286
Stopping Sample Acquisition 286
Using Pause After in an Experiment 286
Stopping and Resuming an Experiment 287
Exporting FCS and RLST Data Files 288
Exporting the FCSData File for a Single Position 288
Exporting FCS and RLST Data Files 290
Chapter 9 Applying Fluorescence Compensation 291
Setting Up the Experiment 291
Adjusting Compensation Automatically 295
Adjusting Compensation Manually 298
Dragging Populations 298
Editing the Compensation Matrix 299
viii | ZE5 Cell Analyzer and Everest Software