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Using High-Throughput Mode
In high-throughput mode, acquisition time is greatly reduced. Wells are sampled continuously with no
boosting to the flow cell in between. In this mode, multiple samples occupy the sample line at the
same time.
To use high-throughput mode, you must use the Experiment Builder to program it into your run list. If
samples have not yet been assigned to high-throughput mode, you can click Edit to modify the run list.
To sample in high-throughput mode
1. Ensure that instrument settings have been configured properly for the experiment setup and
sample type by acquiring control positions in Setup mode as described in Acquiring Initial Sample
in Setup Mode on page 278.
2. Click Acquisition in the Instrument Control panel.
3. Click (Start) Run List.
Acquisition progress is indicated on the positions on the plate. For information about symbols
shown in the plate map, see Plate Map in the Instrument Control Panel on page 205.
Note: Because the sample line contains multiple samples and sample is not boosted to the
flow cell, there is a delay between sample aspiration and the time that sample reaches the
flow cell; this delay depends on the flow rate used in the experiment.
4. Acquisition proceeds until the last programmed well has been acquired.
5. To stop acquisition at any time, click (Stop) Run List.
User Guide | 285
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