Chapter 2 Hardware Description
The weight of each bottle is continuously monitored so the system can calculate the approximate
remaining run time for each bottle. The system automatically switches between the two sets of sheath
and waste bottles, and displays an alert when less than 1 hr of run time remains.
Sheath Bottles
The two sheath bottles hold DI water for the system. This solution is also used for washing the sample
probe between runs. These bottles have a blue cap. Each holds 4 L of fluid; together, they provide
about 8 hr of continuous run time.
Note: When refilling these bottles, ensure that the tubing and filter are reinstalled so that they are
in the lower corner of the bottle opposite the cap.
Sheath Additive Bottle
The sheath additive bottle, which has a white cap, contains 250 ml of concentrated balanced salts,
antimicrobial/antifungal agent, and surfactant. This fluid is drip-fed into the internal sheath reservoir on
a continuous basis while the system is running and helps prevent microbial buildup in the system.
28 | ZE5 Cell Analyzer and Everest Software
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