BIO RAD ZE5 - Beam-Shaping Optics; Interrogation; Light Collection

Chapter 2 Hardware Description
n 488nm (blue) at 100mW
n 561nm (yellow green) at 50mW
n 640nm (red) at 100mW
Caution: Laser irradiation can be hazardous. Do not remove system covers, which are
in place for your safety. Only trained personnel should access the exposed laser
beams.
The laser power and shutter can be controlled through Everest Software. This allows you to turn lasers
on and off and to select different laser powers for different experiments.
Beam-Shaping Optics
Each laser includes an integrated beam shaping optic (BSO) assembly to ensure that each individual
beam is delivered to the flow cell in the correct dimensions and focus. Consistent geometry between
lasers optimizes illumination of each analyzed cell and maintains a high degree of measurement
precision.
Interrogation
An interrogation point occurs where each laser beam intercepts the sample in the flow cell. The ZE5
Cell Analyzer system supports up to five spatially separated interrogation points along the core
stream. Upon interrogation, the particles scatter the laser light and generate fluorescent signals.
Light Collection
Collected light can be categorized as either scattered or fluorescent. Scattered light is collected from
two angles relative to the laser beam: immediately in front of the laser (forward) and at approximately
90° relative to the laser (side). Fluorescent light is collected only from the side.
36 | ZE5 Cell Analyzer and Everest Software