BIO RAD ZE5 - Accessing the Loader

Accessing the Loader
Test Adjustment Event Rate Tests whether the system can acquire beads at 500 events per
second.
Perform Automatic PMT
Adjustment
Adjusts PMT voltages so that the mean value of the bead population
in all fluorescence channels falls into data channel 128.
Perform Automatic Laser Delay
Adjustment
Automatically sets laser delays so that the relevant signals are
coordinated and attributed to the correct cells.
Collect Validation Data Adjusts the event rate to 150 events per second and collects a
5,000-event file.
Save Results Stores data, voltages, and CVs.
Reset Defaults Resets all voltages so that you can proceed with your experiment.
Table 32. Steps in the QC process, continued
The QC workspace loads automatically regardless of whether it was initiated from the Home window
or Acquisition workspace. When QC completes, the software remains in the Acquisition workspace. To
return to the Home window, click the Home button.
Note: The Home button on the toolbar is disabled during the QCprocess. You can return to the
Home window after the QC process completes.
After the QC process is complete, you can access the QC report from the Reports section of the
toolbar.
For more information about reports, see Quality Control and ZE5-EYE Reports on page 313.
Accessing the Loader
To open the loader door and extend the loader
u Do one of the following:
n Press the silver sample chamber button on the front of the instrument.
n Click the Door toggle in the toolbar.
User Guide | 159