Chapter 9 Applying Fluorescence Compensation
Everest™ Software offers simplified workflows for both manual and automatic compensation. To
perform automatic compensation, acquire single-color controls using the Everest compensation
template. You can then adjust regions to define the positive populations in all controls’ channels and
initiate the automatic compensation calculation. Everest Software also offers automatic region
determination, so that you do not need to manually adjust the gates to define the positive populations.
Tip: Everest Software permits visualization of both compensated and uncompensated data in the
same workspace. Be sure to select the Comp parameter for plot axes so that the compensation
matrix is applied to the data.
For information on applying compensation to multicolor experiments, see Appendix B, Example 9-
Color Immunophenotyping Experiment.
Setting Up the Experiment
Before applying compensation in Everest Software (either automatically or manually), set up the
workspace to streamline the compensation process. For information about the Compensation
template, see Setting Up Compensation Controls on page 182.
1. Set up an experiment by selecting fluorophores and activating detectors as described in Chapter
7, Creating Experiments and Workspaces.
User Guide | 291
To Next Page
Loading...
