Fluidics System
Each waste bottle has two connections: one allows waste fluid to pass into the bottle, and the other
allows air to flow out of the bottle when it is displaced by fluid. Each sheath (DI water) bottle has one
connection to the instrument.
The connections for the Additive bottle and System Cleaner bottle are located at the bottom of the
fluidics chamber next to each bottle.
During system operation, the bottles illuminated in green are in use; unlit bottles are not in use and
can be swapped. Examples are shown in the next figure.
For information about connecting the DI water and waste bottles to house DI water and waste,
respectively, see Appendix C, Optional External DI Water and Waste Ports.
Sample Loader
The ZE5 Cell Analyzer features a sophisticated autoloader that can sample from a wide variety of
media types, including racks of forty 5 ml tubes (12 x 75 mm), twenty-four 1.5 ml tubes, 96-well plates
(deep-well or standard depth), and 384-well plates. A single sample position for a 5 ml tube is also
provided for stat tube samples. Additionally, you can use Everest Software to set up custom sample
input devices. The next figure shows the probe sampling a well of a 96-well plate.
User Guide | 31
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