Chapter 12 Maintenance
Required Materials
To complete the decontamination process you will need the following materials.
n Four extra sheath bottles
n Two extra additive bottles
n Two extra cleaner bottles
n Two standard fluorescence-activated cell sorting (FACS) tubes (12 x 75 mm, 5.0 ml)
n 12 L filtered DI water
n 100 ml filtered bleach (filtered through a 0.2 µl filter flask or syringe filter)
Preparing for the Decontamination Process
Important: When handling sheath fluid and DI water bottles, always wear gloves and minimize air
exposure to help avoid contamination.
Before beginning the decontamination process you must
n Decontaminate the outside of the probe with 10% bleach solution.
n Prepare bleach and rinse bottles for decontamination.
n Replace both sheath bottles with bottles filled with 2.5 L diluted 1% bleach.
326 | ZE5 Cell Analyzer and Everest Software
To Next Page
