Table of Contents
Unclogging the Sample Line and Probe 322
Cleaning the Sample Line and Probe 324
Decontaminating the System 325
Required Materials 326
Preparing for the Decontamination Process 326
Decontaminating the System 328
Cleaning the Optical Filters 334
Replacing the QC Beads 335
Maintaining the System When Not in Use 338
Appendix A Troubleshooting 339
Exporting and Viewing Log Files 339
Deleting Acquired Data Files 340
Viewing Everest Software 2.0 FCSFiles in FlowJo Software 342
Fluidics Issues 343
Software Issues 343
Acquisition/Event Issues 344
Hardware/Electronics/Laser Issues 358
Appendix B Example 9-Color Immunophenotyping Experiment 359
Preparing Controls and Samples 359
Setting Up Fluorophores and Controls 360
Setting Up the Sample and Sampling Parameters 362
Creating Plots for the Experimental Sample 366
Acquiring Initial Data 368
Performing Initial Data Analysis 371
Performing Automatic Compensation 373
Resuming Acquisition 375
Analyzing or Exporting Final Data 376
Appendix C Optional External DI Water and Waste Ports 379
Connecting External DI Water 379
Connecting External Waste 381
Specifying External Port Use 381
x | ZE5 Cell Analyzer and Everest Software
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