Running Quality Control
n Tools section on the Acquisition workspace toolbar
The QC process utilizes single-peak beads conveniently located on board. The beads contain a
mixture of dyes that can be excited by all lasers installed on the ZE5 Cell Analyzer and that can emit in
every fluorescence channel. The level of QC beads is monitored and displayed along with the fluidics
status information.
The process first builds a QC workspace and begins acquiring beads. It then ensures that the system
can acquire the beads at both 150 events per second and 500 events per second. Then, at 500 events
per second, the system automatically adjusts laser delays and PMT voltages so that the mean
intensity of the bead population falls into data channel 128 K in all fluorescence channels. CV
increases and PMT voltage changes are compared against the QC criteria. Finally, a 5,000-event file
is collected at 150 events per second and voltages and CVs are stored for reporting purposes. These
values can be displayed, based on the date the process was run, in daily QC reports and QC trending
reports.
Important: While QCis in progress, the following functions are disabled:
n Returning to the Home window
n Initiating the ZE5-EYE process
n Sending the run list to the local instrument (performed in the Analysis workspace)
n In the Instrument tools:
o Returning sample probe to home position
o Cleaning the sample line and probe
o Unclogging the sample line, probe, and flow cell
o Swapping QCbeads
o Pausing the sheath fluid and disabling the lasers
n Opening the sample loader door
The software enables these functions when the QCprocess completes.
While QC is in progress, click the down arrow to display the QC Details window, which provides real-
time updates on each portion of the process.
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