Chapter 6 Daily Routine
Setting Cleaner Pressure Sets cleaner pressure to 12 psi for subsequent steps using cleaner.
Approximately 7.5ml of cleaner is used in the shutdown process.
Flushing Flow Cell with Cleaner Homes the loader; the flow-cell cleaner port opens and the cleaner
pump fills the flow cell with cleaner.
Flushing Flow Cell and Sample
Line with Cleaner
The sample pump runs backwards to move cleaner through the flow
cell and sample lines.
Setting Cleaner for Wash Flush Sets cleaner pressure to the same as the rinse pressure, which is
typically 10 or 12 psi.
Flushing Flow Cell with Sheath Flushes the flow cell with DI for 10 sec.
Flushing Flow Cell and Sample
Line with Sheath
The sample pump runs backwards to flush the flow cell and sample
line with cleaner for 20 sec.
Washing Probe with Cleaner Moves the loader to a safe position, moves the sample probe up and
down between home and the maximum position through the wash
station several times to clean the exterior of the probe with cleaner.
Probe crash is also checked during this step.
Purging Wash Lines Purges wash line with DI for 20 sec.
Rinsing Probe with Sheath Moves the loader to safe position, moves the sample probe up and
down between home and the maximum position through the wash
station several times to clean the exterior of the probe with DI. Probe
crash is also checked during this step.
Emptying Wash Detect Port Turns on the sample waste detect valve, empties the wash detect
port. Waste pump evacuates fluid from the wash station. Cleaner and
air pump run backwards to reduce cleaner and sheath pressure.
Waiting for Fluidics Shutdown Depressurizes sheath pressure to 0 psi, turns off all the pumps,
empties the waste detect in case of drips, waits for fluidics to shut
down in 4 min.
Instrument Shutdown Turns off the LEDs, turns off the loader, ZE5 Cell Anaylzer
instrument shuts down.
Table 33. Steps in the shutdown process, continued
164 | ZE5 Cell Analyzer and Everest Software
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