Table of Contents
Exporting Optical Filter Configurations 134
Reverting to the Default Optical Figure Configuration 134
Using the ZE5-EYE to Confirm Filter Choices 135
Installing a Neutral Density Filter 139
Chapter 6 Daily Routine 141
System Power 141
Starting Everest Software 142
Checking Fluidics Status 142
Refilling Bulk Fluidics 143
Emptying Waste Bottles 145
Replacing Sheath Bottles 146
Replacing Sheath Additive and Cleaner Bottles 148
Logging In 151
Starting Up the System 152
Running Quality Control 156
Accessing the Loader 159
Running Experimental Samples 160
Pausing the System 160
Shutting Down 161
Scheduling Automatic Startups 165
Exiting Everest Software 166
Chapter 7 Creating Experiments and Workspaces 167
Creating a New Experiment 167
Selecting Fluorophores 168
Setting Up the Run List 170
Selecting the Media Type 170
Creating a Custom Media Type 173
Plate Settings Controls 179
Setting the Run List Order 180
Activating Temperature Control 180
Specifying Shutdown Upon Completion 181
Setting Up Compensation Controls 182
Labeling Positions Manually 183
Labeling Positions Automatically 184
vi | ZE5 Cell Analyzer and Everest Software
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