CONTENTS
2-2-2-4 XYZ Observation Mode....................................................................................2-41
2-2-2-5 XYT Observation Mode....................................................................................2-46
2-2-2-6 XYZT Observation Mode .................................................................................2-49
2-2-3 Differences in Image Acquisition Method Between Fluorescent and
Transmitted Images ...................................................................................2-56
2-2-3-1 Monochrome Image .........................................................................................2-56
2-2-3-2 Dual-Fluorochrome Image ...............................................................................2-60
2-2-3-3 Transmitted Image...........................................................................................2-63
2-2-4 Image Acquisition by Rotating It (Rotation Scan) .......................................2-67
2-2-5 Image Acquisition of Only the Rectangular Position (Clip Scan) ................2-68
2-2-6 Image Acquisition by Magnifying the Rectangular Position (Zoom-In Scan)2-70
2-2-7 High-Speed Image Acquisition...................................................................2-73
2-2-8 Image acquisition to prevent crosstalk between fluorescence (Sequential
Scan) .........................................................................................................2-74
2-2-8-1 Virtual Channel Function..................................................................................2-77
2-2-9 Image Acquisition of a Line at Desired Angle.............................................2-82
2-2-10 Display the change of image intensity (Point Scan) .................................2-85
2-2-11 Image Acquisition on Desired Line (XZ, XT or XZT Observation) ............2-89
2-2-12 Image Acquisition in the Laser Excitation Mode .......................................2-92
2-2-12-1 Making REX Mask File...................................................................................2-93
2-2-12-2 Example of FRAP experiment........................................................................2-98
2-3 Saving, Opening and Shredding Images ...................................2-107
2-3-1 Saving Images .........................................................................................2-110
2-3-1-1 Saving Images As a Series............................................................................2-110
2-3-1-2 Saving a Display.............................................................................................2-112
2-3-1-3 Saving Specified Area of Image.....................................................................2-115
2-3-1-4 Saving Animation images ..............................................................................2-118
2-3-1-5 File Types Available for Save.........................................................................2-120
2-3-2 Opening Previously Saved Images ..........................................................2-128
2-3-3 Shredding Images....................................................................................2-129
2-3-4 Saving Comment Together with Image ....................................................2-131
2-3-5 Checking the Image Information/Acquisition Parameters.........................2-133
2-3-6 Saving the Image Information/Observation Condition ..............................2-137
2-3-7 Saving/Reading the Region File...............................................................2-140
2-3-7-1 Saving the Region File ...................................................................................2-140
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