RADIOMETER ABL90 FLEX User Manual

Contents ABL90 FLEX reference manual
Measuring sequence..................................................................5-29
Calculations.............................................................................5-29
Calibration of the pO
2
sensor .............................................................5-30
Introduction.............................................................................5-30
Sensitivity ...............................................................................5-30
Status.....................................................................................5-30
Measurement - pO
2
..........................................................................5-31
Checks....................................................................................5-31
Metabolite sensors ...........................................................................5-32
Construction of the metabolite sensors................................................5-33
Basic description.......................................................................5-33
Diagram..................................................................................5-33
Parts and description.................................................................5-33
Zero current ............................................................................5-33
Calibration of the metabolite sensors ..................................................5-34
Sensitivity ...............................................................................5-34
Measurement – metabolites...............................................................5-35
Measurement...........................................................................5-35
Checks....................................................................................5-35
Measuring principle of the metabolite sensors ......................................5-36
Amperometric measuring principle ..............................................5-36
Electrode chain.........................................................................5-36
Parts and functions ...................................................................5-36
Measuring process ....................................................................5-37
ctHb and derivates ..........................................................................5-38
General information..........................................................................5-39
Measured parameters................................................................5-39
Construction ............................................................................5-39
Measurement cycle ...................................................................5-40
Lambert-Beer's law...................................................................5-40
Absorbance..............................................................................5-41
Total absorbance ......................................................................5-41
Continuous spectrum ................................................................5-41
Spectrum examples ..................................................................5-42
Determining concentrations........................................................5-42
Matrix of constants ...................................................................5-43
Calibration of the optical system ........................................................5-44
Calibration materials.................................................................5-44
Zero point ...............................................................................5-44
Cuvette path length ..................................................................5-44
tHb calibration frequency...........................................................5-44
Correcting for interferences ...............................................................5-45
HbF versus HbA........................................................................5-45
Deviation of results...................................................................5-45
Detecting HbF ..........................................................................5-45
Correcting for HbF ....................................................................5-45
Repressing spectra....................................................................5-45
Residual spectrum ....................................................................5-46
Measurement and corrections ............................................................5-47
Oximetry parameters ................................................................5-47
Bilirubin ..................................................................................5-47
Restrictions..............................................................................5-48
Corrections for ctHb..................................................................5-49
Corrections for ctBil ..................................................................5-49
References......................................................................................5-50
6. User-defined corrections..................................................................6-1

RADIOMETER ABL90 FLEX Specifications

General

Type	Blood Gas Analyzer
Analysis Time	35 seconds
Parameters Measured	pH, pCO2, pO2
Sample Volume	65 μL
Connectivity	Ethernet, USB
Calibration	Automatic
Data Management	Integrated data management system
Sample Type	Whole blood, plasma, serum
Power Requirements	100-240 V AC, 50-60 Hz, 150VA
Operating Temperature	15-32 °C (59-90 °F)
Operating Humidity	20-85% relative humidity, non-condensing