5. Sensors and optical system ABL90 FLEX reference manual
5-36
Measuring principle of the metabolite sensors
The cGlu and cLac sensors are measured according to the amperometric
measuring principle, in which the magnitude of an electrical current flowing
through an electrode chain is related to the concentration of a substance being
oxidized or reduced at an electrode in the chain.
The electrode chain set up to measure glucose/lactate is illustrated in the
following diagram
1
:
The electrode chain describes the electrical circuit consisting of the following:
Item Part Description
1 Amperemeter Measures the current flowing through the circuit in
nanoamperes.
2 Cathode Negative electrode where a reduction reaction
occurs and electrons are consumed.
3 Membrane Allows the appropriate molecules to pass through
from the sample.
4 Sample Contacts the membrane.
5 Electrolyte Provides electrical contact between the anode and
cathode.
6 Anode Positive electrode where an oxidation reaction
occurs and electrons are released.
7 Applied voltage Applies the necessary potential for the reduction or
oxidation reaction under study.
1
Note that polarization voltage is applied between the anode and the reference
electrode (not shown). The current runs through the anode and cathode chain.
Amperometric
measuring
principle
Electrode chain
Parts and
functions
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