RADIOMETER ABL90 FLEX - Page 190

7. Performance characteristics ABL90 FLEX reference manual
7-4
Parameter Primary Reference Method
Secondary
Reference
Method
Reference
cGlu Spectrophotometry, using the
hexokinase (HK) method
recommended by NCCLS, measured
on serum.
N/A [Ref. 5]
cLac Spectrophotometry using a lactate
dehydrogenase (LDH) method,
measured on serum.
N/A [Ref. 8]
ctHb HiCN method recommended by
NCCLS.
ABL735 [Ref. 6]
sO
2
Tonometry:
100%: whole blood is tonometered
with a gas mixture containing
94.4% O
2
and 5.6% CO
2
. 0%:
whole blood is tonometered with a
gas mixture containing 94.4% N
2
and 5.6% CO
2
+ dithionite.
ABL735
FO
2
Hb Measured according to the following
relation:
FO
2
Hb = 1 - (FHHb + FCOHb +
FMetHb)
ABL735
FHHb 0%: whole blood is tonometered
with a gas mixture containing
94.4% N
2
and 5.6% CO
2
+
dithionite.
ABL735
FCOHb Gas chromatography: The
Standards are carbon monoxide
mixtures with atmospheric air,
whose purity is validated in
accordance with NIST SRM 1678 (50
ppm CO in N
2
)
ABL735
FMetHb Spectrometry, modified Evelyn-
Malloy method.
ABL735 [Ref. 7]
ctBil The reference method for total
bilirubin is a spectrophotometric
method (wet chemistry based on a
method from Bayer Healthcare,
Tarrytown USA).
The method is traceable to NIST
SRM916a Bilirubin.
ABL735
FHbF The reference method is based on
Cation Exchange HPLC.
ABL735 [Ref. 15]
General reference: [Ref. 10].