Appendix – Traceability to the primary standards at Radiometer ABL90 FLEX reference manual
II-6
Radiometer measurements
The Hct measurement is based on conductivity measured in a sample and then
corrected for the presence of sodium ions. A Sigma 201 Micro hematocrit
centrifuge with RCF of 12620*g, which fulfills most of the CLSI requirements,
has been used for the test together with 75 mm Microhematocrit capillary tubes
with an inner diameter between 1.1 and 1.2 mm. The centrifugation time has
been 5 minutes.
The conductivity and sodium concentration has been measured on
approximately 1000 blood samples with a sodium concentration varying from 80
mmol/L to 180 mmol/L. Hct measurements have then been correlated to the Hct
measured by the reference method.
The primary working standard is a whole-blood sample. The total bilirubin is
determined on a serum sample prepared from this. The determination is
performed using a Hitachi 717 wet-chemistry analyzer, which uses the
Boehringer Mannheim reagency kit, DPD method, given in Ref. 11. The
reference instrument is calibrated using four levels of NIST SRM916a
unconjugated bilirubin standard material.
ctBil
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