14-40 Sysmex XP-300
Technical Information
Revised October 2012
2. PLT Histogram
Platelet histogram is analyzed using 3 discriminators: 2 discriminators (LD) and (UD) - determined
automatically between 2 - 6 fL and between 12 - 30 fL, respectively -and the fixed discriminator at 12
fL. Regarding PLT histogram, check is made to see that there are no relative frequency errors at
discriminators (LD) and (UD), distribution width error, and there is a single peak.
1) PDW (PLT Distribution Width)
PDW is the distribution width on 20% frequency level with the peak taken as 100%. The unit
applied is fL (femto-liter = 10
-15
L).
• Definitions of PDW and P-LCR
2) MPV (Mean Platelet Volume)
MPV is calculated by the following formula:
Where PCT (%) represents the value weighted with PLT frequency and is called plateletcrit or
platelet volume ratio. The analysis method used is the same as mentioned in HCT analysis principle
“RBC/PLT analysis flow” in “CBC analysis”.
3) P-LCR (Large Platelet Ratio)
This is the ratio of large platelets exceeding 12 fL discriminator and is calculated as the ratio of
the particle count between the 12-fL fixed discriminator and Upper discriminator (UD) to the
particle count between Lower discriminator (LD) and Upper discriminator (UD).
100%
20%
P-LCR
(LD) (UD)(12fL)
PDW
MPV(fL) = ×10,000
PCT(%)
PLT(×10
3
/µL)
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