Acquisition/Event Issues
Error Possible causes Troubleshooting steps
Excessive light leaking into trigger
channel
This typically results in a
BLR (baseline restoration)
fault. Excessive light being
introduced into the trigger
channel causes the baseline
in that detector to move up,
which can drown out signal in
that channel.
This can be caused by high
background fluorescence in
the sample. Run the cleaning
process, using bleach. See
Cleaning the Sample Line
and Probe on page 324.
If this does not resolve the
problem, contact Bio-Rad
Technical Support for
assistance.
Flow cell dirty Ensure that the flow cell is
clean. Built-up cellular
material on the cuvette walls
can cause unexpected light
scatter, which can then be
introduced into the scatter
detector inappropriately. Run
the unclog process in
Everest Software to clean the
flow cell. See Unclogging the
Sample Line and Probe on
page 322.
User Guide | 345
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